Induction of Tumor Necrosis Factor (TNF) Release from Subtypes of T Cells by Agonists of Proteinase Activated Receptors
Figure 4
Effect of PD98059 and LY294002 on release and gene expression of TNF in purified T cells. PD98059 (PD, 50 μM) or LY294002 (LY, 20 μM) was preincubated with T cells at 37°C for 30 min before 3.0 μg/mL of thrombin and 0.3 μg/mL of trypsin being added for 6 or 16 h. (a) Cells were collected for analysis of TNF release from T cells at 16 h following incubation. (b) Cells were collected for analysis of gene expression of TNF in T cells at 6 h following incubation. Values shown are mean ± SEM for four to six independent experiments from different donors. * compared with the response to medium alone control. † compared with the response to the corresponding proteinase alone. Th = thrombin (μg/mL), Ts = trypsin (μg/mL), LY = LY294002 (μM), and PD = PD98059 (μM).
