p38/AP-1 Pathway in Lipopolysaccharide-Induced Inflammatory Responses Is Negatively Modulated by Electrical Stimulation
Figure 5
Effect of SB203580 on the expression of COX-2 and TNF- mRNA and the production of TNF- and PGE2. ((a) and (b)) The levels of COX-2 and TNF- mRNA were determined using semiquantitative RT-PCR or real-time PCR. (a) The levels of TNF- and PGE2 were determined using ELISA and EIA on culture supernatants from RAW264.7 cells that had been treated with SB203580 (20 M) and LPS (1 g/mL) for 6 h (TNF-) or 24 h (PGE2) h. * and ** compared to control.