Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth Restriction

Table 2

(a) Function and functional relationship analysis of target genes of differentially expressed extracellular C19MC microRNAs in preeclampsia in relation to immune system response. (b) Function of target genes of differentially expressed extracellular C19MC microRNAs in preeclampsia in immune system response.

(a)  

microRNA	miR-516-5p	miR-517*	miR-526a	miR-525	miR-520a*
Number of predicted target genes	349	179	212	340	352
	Unique target genes				Shared with miR-525
	CCR2	FAS	BCAP29	TOX	ACVR2B
	CD109	IL6ST	CD24		AHSA2
	CD1A	IL9R	CD302		ATRN
	DNAJC25	IRAK3	CFLAR		CD2
	FLT1	LILRA2	DNAJC21		CD300LB
	IL17RE	MTDH	HSP90AA1		CD46
	IRAK1	PAPPA	IGFBP1		CD93
	LILRB5		TLR2		HSF5
	PDCD6IP		TNFRSF19		IGF1R
	SOCS2		TNFSF15		IL10RA
			TRAF6		MMD2
					PPARA
					TLR7
					VSIG4

All the targets were predicted by a bioinformatics tool MirTarget2 using miRDB online database.

(b)  

Gene official symbol	Gene full name	The role in immune system response
ACVR2B	Activin A receptor, type IIB	Activins belong to the TGF-𝛽 superfamily
AHSA2	AHA1, activator of heat shock 90 kDa protein ATPase homolog 2 (yeast)	Hsp90 is an inducible molecular chaperone protecting stressed cells
ATRN	Attractin	Involvement in initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines
BCAP29	B-cell receptor-associated protein 29	Involvement in CASP8-mediated apoptosis
CCR2	Chemokine (C-C motif) receptor 2	Binds monocyte chemoattractant protein-1 involved in monocyte infiltration during inflammation
CD109	CD109 molecule	Encodes GPI-linked glycoprotein that negatively regulates signaling of TGF-𝛽
CD1A	CD1a molecule	Encodes glycoproteins structurally related to MHC proteins mediating the presentation of lipid and glycolipid antigens
CD2	CD2 molecule	A surface antigen of thymocytes, T, and NK cells
CD24	CD24 molecule	Encodes a sialoglycoprotein expressed on mature granulocytes and B cells
CD300LB	CD300 molecule-like family member b	A nonclassical activating receptor of the Ig superfamily expressed on myeloid cells
CD302	CD302 molecule	A C-type lectin receptor involved in cell adhesion, migration, endocytosis, and phagocytosis
CD46	CD46 molecule, complement regulatory protein	Has cofactor activity for inactivation of complement components C3b and C4b by serum factor I
CD93	CD93 molecule	Involvement in intercellular adhesion and in the clearance of apoptotic cells
CFLAR	CASP8 and FADD-like apoptosis regulator	Regulator of apoptosis structurally similar to caspase-8
DNAJC21	DnaJ (Hsp40) homolog, subfamily C, member 21	A molecular chaperone protein protecting against cellular stress
DNAJC25	DnaJ (Hsp40) homolog, subfamily C, member 25	A molecular chaperone protein protecting against cellular stress
FAS	FAS cell surface death receptor (FAS)	Plays a central role in regulation of programmed cell death
FLT1	fms-related tyrosine kinase 1	A member of vascular endothelial growth factor receptor (VEGFR) playing an important role in angiogenesis and vasculogenesis
HSF5	Heat shock transcription factor family member 5	A transcriptional activator of heat shock genes
HSP90AA1	Heat shock protein 90 kDa alpha (cytosolic), class A member 1	An inducible molecular chaperone protecting stressed cells
IGF1R	Insulin-like growth factor 1 receptor	Antiapoptotic agent enhancing cell survival
IGFBP1	Insulin-like growth factor binding protein 1	Prolongs the half-time of IGFs in plasma that regulate cell growth and development
IL10RA	Interleukin 10 receptor, alpha	Involvement in inhibition of the synthesis of proinflammatory cytokines
IL17RE	Interleukin 17 receptor E	Participation in MAPK pathway
IL6ST	Interleukin 6 signal transducer (gp130, oncostatin M receptor)	A signal transducer shared by IL-6, LIF, and oncostatin M
IL9R	Interleukin 9 receptor	Mediates IL-9 effects like stimulation of cell proliferation and prevention of apoptosis
IRAK1	Interleukin-1 receptor-associated kinase 1	Responsible for IL-1 induced upregulation of the transcription factor NF-kappa B
IRAK3	Interleukin-1 receptor-associated kinase 3	Functions as a negative regulator of Toll-like receptor signaling
LILRA2	Leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2	An activatory cell-surface receptor expressed on monocytes, B cells, dendritic, and NK cells
LILRB5	Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 5	An inhibitory cell-surface receptor expressed on immune cells
MMD2	Monocyte to macrophage differentiation-associated 2	Modulates Ras signaling
MTDH	Metadherin	Involvement in HIF-1 alpha mediated angiogenesis and RNA-induced silencing complex and miRNA functions
PAPPA	Pregnancy-associated plasma protein A, pappalysin-1	Involvement in local proliferative processes such as wound healing
PDCD6IP	Programmed cell death 6 interacting protein	Protects against cell death
PPARA	Peroxisome proliferator-activated receptor alpha	Affects the expression of genes involved in cell proliferation, cell differentiation, and in immune and inflammation responses
SOCS2	Suppressor of cytokine signaling 2	A negative regulator of JAK/STAT cytokine signaling pathway
TLR2	Toll-like receptor 2	Plays a fundamental role in activation of innate immunity, stimulates NF-kappa B
TLR7	Toll-like receptor 7	Plays a fundamental role in activation of innate immunity
TNFRSF19	Tumor necrosis factor receptor superfamily, member 19	Interacts with TRAF family members, induces apoptosis by a caspase-independent mechanism
TNFSF15	Tumor necrosis factor (ligand) superfamily, member 15	A cytokine induced by TNF and IL-1 alpha activating NF-kappa B and MAP kinases inducing apoptosis in endothelial cells
TOX	Thymocyte selection-associated high mobility group box	Highly expressed in thymus, the site of development of T cells
TRAF6	TNF receptor-associated factor 6, E3 ubiquitin protein ligase	Functions as a signal transducer in the NF-kappa B pathway, activates Ikappa B kinase in response to proinflammatory cytokines
VSIG4	V-set and immunoglobulin domain containing 4	A negative regulator of T-cell responses structurally related to the B7 family of immune regulatory proteins