The Serum IL-6 Profile and Treg/Th17 Peripheral Cell Populations in Patients with Type 1 Diabetes
Figure 2
Comparison between percentage (a) and absolute number (b) of CD4+IL17A+ T cells in peripheral blood of diabetic type 1 patients and control group. PBMCs from diabetic type 1 patients (DM1) and healthy individuals (C) were cultured and stimulated as described in Section 2 and then stained with antibodies against CD4 and IL17A. The percentage of CD4+IL17A+ T cells was determined by flow cytometry. Analyzing CD4+IL17A+ cells, dot plots representing anti-CD4 versus SS were carried out to establish CD4+ and CD4− lymphocyte gates. Then, the anti-CD4 versus IL17A from CD4+ gate dot plot was generated and the frequency of Th17 cells was determined. Data were calculated with Mann Whitney test and presented as medians and minimum/maximum. The median percentage of CD4+IL17A+ T cells in DM1 and control group was 2.25 (0.8/5.4) and 1.3 (0.3/2.9), respectively. The median absolute number of CD4+IL17A+ T cells in DM1 and control group were 0.49 (0.9/1.8) and 0.34 (0.06/0.59), respectively. *The percentage of cells among peripheral blood lymphocytes.
