ATP Is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro
Figure 2
IL-6, intracellular calcium chelation, and P2X inhibition prevent the induction of gap junctional communication promoted by TNF-α plus ATP or TNF-α/IFN-γ. (a) Graph showing the effect of acutely applied 50 μM 18-β-glycyrrhetinic acid (β-GA) or pretreatment with 10 ng/mL interleukin-6 (IL-6), 300 μM oxidized ATP (oATP), or 10 μM BAPTA-AM on the incidence of dye coupling (IDC) of microglia treated for 3.5 h with TNF-α plus ATP. (b) Graph showing the effect of 50 ng/mL IL-6 or 300 μM oATP over the IDC of microglia treated for 9 h with TNF-α/IFN-γ. Data is expressed as a percentage of IDC under control conditions (dashed line). versus control condition. Each bar represents the mean ± SEM, . No significant differences were observed when comparing microglia and EOC20 cells responses to different treatment in dye transfer assays.
