In Vivo TLR9 Inhibition Attenuates CpG-Induced Myocardial Dysfunction

Figure 3

(a)–(f) RT-qPCR of mRNA expression of proinflammatory cytokines TNF-α, IL-1β, and IL-6 in the hearts of WT and TLR9-D mice measured 2 and 6 h after application of 1668-thioate. The control ODN 1612-thioate or the TLR9 inhibitors H154- and IRS954-thioate as well as chloroquine were injected 30 min after stimulation. PBS application served as control, and TLR9-D animals (last bar) were stimulated with 1668-thioate + PBS as negative control (mean ± SEM; 𝑛=8/group, *𝑃<0.05; *also indicates the significant group).

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