Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Figure 5
Protein levels of cytokines and cardiomyocyte contractility. (a), (b) Protein levels of proinflammatory cytokines were analysed via ELISA 18 h after CASP in hearts of WT Sham, WT CASP, and TLR9-D CASP mice (*:; ; mean ± SEM). (c), (d) Original recordings of sarcomere shortenings of isolated cardiomyocytes incubated in culture medium (CM) or culture medium + heat-inactivated bacteria (HIB) stimulated at 4 Hz. (e) Plot of averaged sarcomere-shortening amplitude versus stimulation frequency recorded directly after isolation (WT control) or after incubation with culture medium (WT CM) or with CM + HIB (#: WT HIB versus WT control; *: WT HIB versus WT CM; ; mean ± SEM). (f) Comparison of sarcomere-shortening amplitude at 4 Hz stimulation frequency of WT control cardiomyocytes and WT and TLR9-D cardiomyocytes incubated in HIB for 5–7 h (; *:; mean ± SEM).
