Modulation of Murine Macrophage TLR7/8-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium
Figure 1
Modulation of cytokine expression by BMDMs cocultured with MSCs and then stimulated with TLR7/8 ligands. BMDMs (0.4 × 106 cells/well) were incubated with or without MSCs (0.4 × 106 cells/well) in 24-well plates. After 1 h, the cells were incubated with R848 (TLR7/8 ligand, 10 μg/mL) or loxoribine (TLR7 ligand, 300 μg/mL) for 24 h. The levels of murine TNF-α, IL-6, GM-CSF, IL-12 p70, and IL-10 in the culture supernatant were determined using ELISA. Data are expressed as the mean ± SEM. in each group. and , compared to the control group. N.D., not detected. Results are representative of 3 independent experiments.
