Modulation of Murine Macrophage TLR7/8-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium
Figure 2
Modulation of cytokine expression by BMDMs incubated in MSC-conditioned medium and then stimulated with TLR7/8 ligands. BMDMs were preincubated for 1 h in MSC-conditioned medium or control medium and then incubated with R848 (TLR7/8 ligand, 10 μg/mL) or loxoribine (TLR7 ligand, 300 μg/mL) for 24 h. (a–e) The levels of murine TNF-α (a), IL-6 (b), GM-CSF (c), IL-12 p70 (d), and IL-10 (e) in the culture supernatant were determined using ELISA. Data are expressed as the mean ± SEM. in each group. and , compared to the control medium group. N.D., not detected. (f–i) The levels of Il1b (f), Cxcl1 (g), Ccl3 (h), and Ccl5 (i) mRNA were determined using qRT-PCR. Data are expressed as fold increase over the level in the control medium group without TLR7/8 ligand stimulation (mean ± SEM). in each group. and , compared to the control medium group. Results are representative of 3-4 independent experiments.
