Modulation of Murine Macrophage TLR7/8-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium
Figure 3
Contribution of PGE2/EP to the modulation of cytokine expression in BMDMs incubated in MSC-conditioned medium and then subjected to R848 stimulation. (a) PGE2 levels in MSC-conditioned medium or control medium were measured by ELISA. N.D., not detected. (b–d) BMDMs were incubated in MSC-conditioned medium or control medium with EP2 antagonist (AH6809, 10 μM) or EP4 antagonist (GW 627368X, 10 μM). DMSO was used as the vehicle for each EP antagonist. After 1 h, R848 (TLR7/8 ligand, 10 μg/mL) was added, and the BMDMs were incubated for an additional 24 h. The levels of TNF-α (b), IL-6 (c), and IL-10 (d) were determined using ELISA. , compared to the control medium group. , compared to the R848-stimulated cells (without EP antagonist). Data are expressed as the mean ± SEM. in each group. Results are representative of 3 independent experiments.
