Modulation of Murine Macrophage TLR7/8-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium
Figure 4
Enhanced ERK and suppressed NF-κB signaling in BMDMs incubated in MSC-conditioned medium and then subjected to R848 stimulation. BMDMs were preincubated for 1 h in MSC-conditioned medium or control medium, after which R848 (TLR7/8 ligand, 10 μg/mL) was added. Cells were further incubated for the indicated times. (a) The levels of p-p38, p-JNK, and p-ERK were determined by immunoblotting. Beta-actin was used as a loading control. Relative band intensities ware quantified by densitometry analysis. (b) The data of (a) at 0.5h after R848 stimulation obtained by densitometric analysis are shown as the mean ± SEM of 3 independent experiments. (c) The level of total IκB-α was determined by immunoblotting. Beta-actin was used as a loading control. Relative band intensities ware quantified by densitometry analysis. (d) The data of (c) at 1 h after R848 stimulation obtained by densitometric analysis are shown as the mean ± SEM of 3 independent experiments. , compared to the control medium group.
