Research Article

The Endothelial Tyrosine Phosphatase SHP-1 Plays an Important Role for Vascular Haemostasis in TNFα-Induced Inflammation In Vivo

Figure 1

Inhibition of SHP-1 leads to increased platelet rolling in vivo in TNFα-induced systemic inflammation. (a) In vivo the amount of rolling platelets (defined as platelets with a velocity of less than 5% of maximal velocity) as analyzed by intravital microscopy in the dorsal skinfold chamber was slightly enhanced in vivo after treatment with the SHP-1 inhibitor sodium stibogluconate (10 μg/mL, 30 min) under basal conditions but significantly increased in TNFα-induced inflammation (TNFα 5 ng/mL, 4 h). (b) Inhibition of SHP-1 by sodium stibogluconate in TNFα-induced inflammation resulted in a leftward shift in platelet velocity distribution pattern (i.e., towards lower platelet velocities) in the frequency histogram, indicating increased transient platelet interaction with the endothelium. The histograms display all platelet velocities from 5 different animals per group. SG: sodium stibogluconate. *Significantly different at ( platelets per group from 5 different animals).
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(a)
279781.fig.001b
(b)