Pharmacological Inhibition of p38 Mitogen-Activated Protein Kinases Affects KC/CXCL1-Induced Intraluminal Crawling, Transendothelial Migration, and Chemotaxis of Neutrophils In Vivo
Figure 5
Effect of SB203580 on neutrophil Mac-1 expression in vitro. (a) Original histogram of Mac-1-dependent fluorescence in bone-marrow-derived neutrophils in the absence (gray shadow) or in the presence of stimulation with KC (5 nM for 10 min; black line). Neutrophils treated with p38 MAPK inhibitor SB203580 (10 μM for 40 min) are shown as a dotted line. (b) Means ± SEM () of Mac-1-dependent fluorescence expressed as geomeans in unstimulated neutrophils (control; white bar) and in neutrophils stimulated with KC (black bar) and with KC plus SB203580 (gray bar). ** indicates significant difference () from the control without KC stimulation and ## indicates significant difference () from KC stimulation without SB203580 (t-test).
