Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum

Figure 5

GLP-1 restores PDX-1 protein levels in HIT-T15 cells cultured in the presence or absence of AGEs. (a)-(b) Semiquantitative RT-PCR for PDX-1 mRNA expression in HIT-T15 cells cultured 5 days in standard medium (CTR) or in medium containing AGEs (GS) in the presence or absence of 10 nmol/L GLP-1. (a) Representative agarose gel of three different experiments. (b) Quantification of densitometries of agarose gel bands. Data were represented as percentage change from CTR level (100%) (𝑛=3). (c)-(d) Western blot analysis for PDX-1 protein intracellular expression on HIT-T15 cells, cultured in the same conditions of mRNA experiments. (c) Representative western blot of three different experiments. (d) Quantification of densitometries of western blot bands. Data were expressed as mean ± SE of fold induction relative to β-actin (𝑛=3). ^∗∗𝑃<0.01 versus CTR; ^§𝑃<0.05 versus GS.