Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum

Figure 6

Pharmacological inhibition of proteasome increased accumulation of MafA and PDX-1. HIT-T15 cells were cultured for the last 15 h in the presence or absence of the proteasome inhibitor MG-132, and whole cell extracts were analyzed by western blotting using specific antibodies against PDX-1 and MafA. Actin amounts were analyzed as a loading control. ^∗𝑃<0.05, ^∗∗𝑃<0.01 and ^∗∗∗𝑃<0.001 versus CTR or versus the same condition without MG132.

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