Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum

Figure 8

GLP-1 restores proinsulin content in HIT-T15 cells. (a) Semiquantitative RT-PCR for preproinsulin. Representative agarose gel of three different experiments. (b) Quantification of densitometries of agarose gel bands. Data are represented as percentage change from CTR level (100%) (𝑛=3). (c) Intracellular proinsulin was measured after acidified ethanol extraction. Data were expressed as mean ± SE of at least 3 independent experiments. ^∗∗𝑃<0.01 and ^∗∗∗𝑃<0.001 versus CTR; ^§§𝑃<0.01 versus GS.

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