Figure 5: Western blotting for IL-1β and IFN-γ. ((a), (c)) Immunoblots of iNOS−/− control (Cont), cuprizone (CPZ), and cuprizone plus sildenafil (CPZ + Sild) groups. ((b), (d)) Graphs represent quantification and statistical analysis. The control group showed basal expression of IL-1β and IFN-γ. CPZ treatment induced a significant increase of these cytokines, compared to control. Sildenafil plus CPZ significantly decreased IL-1β and IFN-γ expression, in relation to the CPZ group. The experiment was performed in triplicate. The results were expressed as mean ± SE. **, ***, compared to control; ##, ###, compared with CPZ.