Figure 6: Western blotting for eNOS. (a) Immunoblot of wild-type mice control (WT cont), iNOS−/− mice control (iNOS−/− cont), iNOS−/− animals treated with cuprizone (iNOS−/− CPZ), and iNOS−/− animals treated with cuprizone plus sildenafil (iNOS−/− CPZ + Sild). (b) Graph represents quantification and statistical analysis. eNOS was physiologically expressed in WT mice. Animals without iNOS without treatment showed a significant increase of this enzyme, compared to WT control. After CPZ and CPZ plus sildenafil, iNOS−/− animals also showed a significant increase of eNOS, compared to WT animals, but no significant difference was identified between iNOS−/− control and treated animals. The experiment was performed in triplicate. The results were expressed as mean ± SE. *, ***, compared to control.