Prostaglandin D2 in Inflammatory Arthritis and Its Relation with Synovial Fluid Dendritic Cells
Figure 2
Flow cytometric analysis of cells prepared from SF aspirates. (a) Shows the selected gate based on forward and side scatter of light. (b) Shows CD11b staining of 50% of SF cells in a single peak. (c) Shows 3-colour flow cytometric analysis in which 80% of CD11b+ HLADR+ SF cells express CD11c. (d) Shows 3-colour analysis of SF cells in which 20% of CD11b+ HLADR+ cells express CD163. (e) Shows 4-colour analysis to separate CD14− from CD14+ subsets of HLADR+ CD11b+ CD11c+ cells. The CD14− cells were enriched for cells with DC morphology (f), whereas the CD14+ subpopulation displayed monocyte morphology (g).
