Chronic Pain Treatment: The Influence of Tricyclic Antidepressants on Serotonin Release and Uptake in Mast Cells
Figure 5
The influence of selected TCAs on 5-HT content after stimulation of mast cells with different secretagogues. (a) Mast cells were preincubated with increasing concentrations (10−8–10−4 mol/L) of antidepressants (amitriptyline, doxepin, imipramine, and clomipramine) for 10 min and then incubated with compound 48/80 (0.2 μg/mL) for the next 60 min. (b) Mast cells were preincubated with increasing concentrations (10−8–10−4 mol/L) of antidepressants (amitriptyline, doxepin, imipramine, and clomipramine) for 10 min and then incubated with concanavalin A (100 μg/mL) for the next 60 min. Each point represents mean ± SEM of 5 experiments. * versus compound 48/80 (a) or concanavalin (b).
