Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes

Figure 2

Effects of CM on MMP-3 (a) and MMP-13 (b) proteins and mRNA (c) expression in OA chondrocytes. (a, b) Protein levels were measured by ELISA in cell supernatants after 24 h or 5 days of incubation of chondrocytes in the presence or absence of IL-1𝛽 and CM. (c) mRNA expression was determined by real-time PCR analysis in OA chondrocytes after 24 h of incubation in the presence or absence of IL-1𝛽 and CM. Results indicate relative expression with respect to nonstimulated OA chondrocytes. Data represent mean ± S.E.M. of independent cultures with chondrocytes from 4 different donors. ⁺𝑃<0.05, ⁺⁺𝑃<0.01 with respect to nonstimulated cells; *𝑃<0.05, **𝑃<0.01 with respect to IL-1𝛽; ^##𝑃<0.01 with respect to the corresponding group after 24 h incubation.

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