Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes

Figure 4

Effects of CM on the protein levels of IL-6 (a), TNF𝛼 (b), and IL-10 (c) in supernatants and mRNA expression in OA chondrocytes (d). (a–c) Cytokines were measured by ELISA in cell culture supernatants after 24 h or 5 days of incubation of chondrocytes with CM in the presence or absence of IL-1𝛽. (d) mRNA expression was determined by real-time PCR analysis in OA chondrocytes after 24 h of incubation and results indicate relative expression with respect to nonstimulated OA chondrocytes. Data represent mean ± S.E.M. of independent cultures with chondrocytes from 6 different donors. ⁺⁺𝑃<0.01 with respect to nonstimulated cells; *𝑃<0.05, **𝑃<0.01 with respect to IL-1𝛽; ^##𝑃<0.01 with respect to the corresponding group after 24 h incubation.

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