Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes
Figure 6
Effects of CM on NO (a) and PGE2 (b) production and iNOS, COX-2, and mPGES-1 mRNA (c) expression in OA chondrocytes. (a) NO was measured by fluorometry as nitrite. (b) PGE2 was measured by radioimmunoassay. Mediators were determined in cell culture supernatants after 24 h or 5 days of incubation of OA chondrocytes with CM in the presence or absence of IL-1. (c) mRNA expression was determined by real-time PCR analysis in OA chondrocytes after 24 h of incubation and results indicate relative expression with respect to nonstimulated OA chondrocytes. Data represent mean ± S.E.M of independent cultures with chondrocytes from 6 (NO, PGE2) or 4 (mRNA) different donors. ++ with respect to nonstimulated cells; *, ** with respect to IL-1; ## with respect to the corresponding group after 24 h incubation.
