Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone
Figure 1
Characterization of EPC (a–c) and MSC (d, e). Representative results were shown. EPC demonstrated a spindle-shaped appearance (a). The ability of EPC for DiLDL uptake (red color) and the binding of UEA-lectin-FITC (green color) are shown in (b). The image consists of a superimposition of the FITC-fluorescence channel and the Rhodamine-fluorescence channel. Cells carrying both characteristics appear in orange. The protein expression of vWF (c) and CD31 (PECAM, (c)) was assessed by flow cytometry (filled histograms demonstrate the isotype control; black line indicates the antibody staining). The MSCs were characterized by flow cytometry (d); the cells were negative for CD34 and CD45 and express CD73, CD90 (Thy-1), and CD105 (Endoglin, filled histogramms demonstrate the isotype control; black line indicates the antibody staining). In (e) the ability of MSC for osteogenic differentiation was assessed by means of von Kossa staining. The left image demonstrated the calcium deposition (black spots) under control conditions; the right image depicted the calcium deposition under osteogenic culture conditions. Scale bars represent 50 μm (a), 100 μm (b), and 500 μm (e).
