Natural Antioxidant-Isoliquiritigenin Ameliorates Contractile Dysfunction of Hypoxic Cardiomyocytes via AMPK Signaling Pathway

Figure 3

ISL treatment stimulated cardiac AMP-activated protein kinase (AMPK) and ERK signaling pathways. Representative immunoblots of isolated mouse cardiomyocytes showed phosphorylation of (a) AMPK at Thr¹⁷² (p-AMPK), (b) ACC (Ser⁷⁹), and (c) ERK. Phosphorylated AMPK was quantified relative to total AMPKα. Phosphorylated ACC and ERK were quantified relative to GAPDH. Values are expressed as means ± SE (𝑛=3--6), ^∗𝑃<0.05 versus vehicle.

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