Carrageenan-Induced Colonic Inflammation Is Reduced in Bcl10 Null Mice and Increased in IL-10-Deficient Mice
Figure 7
Effects of carrageenan exposure in IL-10-deficient mice compared to C57BL/6 control in germ-free environment and in standard housing. (a) KC was not different in the C57BL/6 germ-free mice versus mice with standard housing at baseline or following carrageenan exposure. In the IL-10-deficient mice, KC was greater at baseline () and following carrageenan () than in the control mice, and the increase in KC was greater in the IL-10-deficient mice than in the controls (~271 ng/L versus ~87 ng/L). (b) Fecal calprotectin was not different in the germ-free mice versus the standard housing control mice with or without carrageenan exposure. Fecal calprotectin was greater in the IL-10-deficient mice at baseline () and following CGN (). (c) Bcl10 was similar in the C57BL/6 mice in the germ-free environment as in standard housing. Baseline Bcl10 was significantly greater in the IL-10 null mice than in the controls (), and increased more in the IL-10-deficient mice (~229% versus ~144%) following carrageenan () than in the control mice. (d) The germ-free environment did not affect the RelA results in the C57BL/6 mice. Baseline RelA was significantly greater in the IL-10 null mice than in the control C57Bl/6 mice () and increased more following carrageenan in the IL-10-deficient mice than in the controls (). (e) Carrageenan-induced increase in RelB was unaffected by IL-10 deficiency. (f) Carrageenan-induced increase in phospho(Ser138)-Bcl10 was unaffected by IL-10 deficiency or the germ-free environment. CGN: carrageenan; N.D.: no difference.
