Carrageenan-Induced Colonic Inflammation Is Reduced in Bcl10 Null Mice and Increased in IL-10-Deficient Mice
Figure 8
In NCM460 cells, exogenous IL-10 inhibits the canonical but not the non-canonical NF-κB pathway that is activated by carrageenan and mediated by Bcl10. (a) Increase in RelA following exposure to carrageenan was partially inhibited by exogenous IL-10 in the NCM460 cells. When Bcl10 was silenced by siRNA, the increase in RelA was reduced and completely inhibited by IL-10 (20 μg/L 24 h). (b) The increase in phospho(Ser32)-IκBα was partially inhibited by exogenous IL-10 in the control and control siRNA cells. The increase was less when Bcl10 was silenced and was almost completely inhibited by exogenous IL-10. (c) In contrast to the above findings, the increase in RelB was unaffected by exogenous IL-10. (d) Phospho(Ser138)-Bcl10 increased to over four times the baseline in the control and control siRNA cells, and the increases were not inhibited by exogenous IL-10. ND: no difference; IL-10: interleukin-10; si: small interfering siRNA; CGN: carrageenan.
