Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation
Figure 3
IR models of 3T3-L1 cell and HepG2 cell were established by hyperinsulinemic method (treated with 0, 0.05, 0.5, 5 and 50 nM insulin for 24 h), then stimulated by 100 nM insulin for 15 min and the fluorescence of 2-NBD labeled glucose was examined. , compared with normal control group (without insulin).
