Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation
Figure 4
The effects of metformin on the secretion and expression of PEDF during IR improvement in vitro. (a) IR models of 3T3-L1 cell and HepG2 cell were treated with metformin (0.01, 0.1, and 1 mM) for 24 h, and 2-NBDG uptake was examined. Finally, 0.1 mM metformin was chosen for later experiments. (b) Supernatants of the cells were collected, and PEDF concentrations were determined by ELISA. (c) RT-PCR assay results of PEDF mRNA levels. (d) Western blot analysis of PEDF protein expression and phosphorylation of AMPK. The histogram represents mean ± SD of the densitometric scans for protein bands from three experiments, normalized by comparison with -actin and expressed as a percentage of control. NC: normal control; IR: insulin resistance; MET: metformin; iA: inhibitor of AMPK. , compared with NC group. , compared with IR group. , compared with MET group.
