Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation

Table 1

Specific primers used for real-time PCR.

Gene	Forward and reverse primers	Amplified fragment (bp)
Actin(rat)	5^′ GCTGTGCTATGTTGCCCTAGAC 3^′	122
	5^′ GGAACCGCTCATTGCCGATAG 3^′
Actin(mouse)	5^′ GCTGTGCTATGTTGCTCTAG 3^′	117
	5^′ CGCTCGTTGCCAATAGTG 3^′
Actin(human)	5^′ ATCGTGCGTGACATTAAG 3^′	135
	5^′ ATTGCCAATGGTGATGAC 3^′
PEDF(rat)	5^′ CGTAGTGGAGGAGGATGAC 3^′	137
	5^′ TGAGAGGAGACAGCAGAATG 3^′
PEDF(mouse)	5^′ CCTCAGCATCCTTCTCCTTGG 3^′	117
	5^′ ACTCTCACGGTCCTGTCCTC 3^′
PEDF(human)	5^′ TTGAGTGGAACGAGGATG 3^′	149
	5^′ CTTGCCAATGAAGAGAAGG 3^′
FAS(rat)	5^′ CGGCGGCAGCAGGAACAG 3^′	86
	5^′ GCACTCTCAGACAGGCACTCAG 3^′
ACC(rat)	5^′ TTGGTGCTTATATTGTGGATGG 3^′	87
	5^′ ATGTGCCGAGGATTGATGG 3^′
G-6-Pase(rat)	5^′ TGGTGGCTGGAGTCTTGTC 3^′	81
	5^′ TCTGGAGGCTGGCATTGTAG 3^′
PEPCK(rat)	5^′ CCCGAAGGCAAGAAGAAATACC 3^′	131
	5^′ TTCATCCAGGCAATGTCATCAC 3^′