Hyperoxia Exacerbates Postnatal Inflammation-Induced Lung Injury in Neonatal BRP-39 Null Mutant Mice Promoting the M1 Macrophage Phenotype

Figure 5

BRP-39 deletion inhibits M2 phenotype in macrophages.  Attenuated expression of M2 marker genes Arg1 and Fizz1 in BRP-39^−/− peritoneal macrophages stimulated with interleukin-4 (IL-4; 10 ng/mL) for 16 h ((a) and (b)). Protein levels (Western blot and densitometry) of M2 markers Arg1 and Ym1 in bone marrow derived macrophages (BMDMs) (c). Results expressed as the mean ± SEM of data obtained from three independent experiments stimulated with IL-4 (10 ng/mL) for 24 h. C: control (RA); HYP: hyperoxia; BRP39 KO: BRP39 knock out or BRP-39^−/−. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001.

(a)

(b)

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