Gestational Exposure to a Viral Mimetic Poly(I:C) Results in Long-Lasting Changes in Mitochondrial Function by Leucocytes in the Adult Offspring
Figure 1
Mitochondrial outcomes in splenocytes. Splenocytes from offspring (10–12 weeks old) poly(I:C)- or saline- treated dams at 12.5 gestational day were isolated, and mitochondrial outcomes were tested as described in detail in Section 2. (a) Oxygen uptake of intact cells was determined using a Clark-type electrode in Hanks balanced salt solution (HBSS) supplemented with 6 mM glucose as substrate. Basal (only glucose), oligomycin (0.2 μM), and FCCP (20 μM) were added, and the initial rates of oxygen uptake were calculated and normalized by million cells. (b) Permeabilized cells were tested for their capability to consume oxygen coupled to ATP production by supplementing the media with malate-glutamate (or NADH oxidase comprised by complexes I–V), succinate with rotenone (or succinate oxidase comprised by complex II–V), and TMP-ascorbate for cytochrome c oxidase activity (or complex IV). (c) ratio of rates of oxygen consumption in the presence of various substrates derived from (b).
