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Mediators of Inflammation
Volume 2013 (2013), Article ID 612848, 7 pages
http://dx.doi.org/10.1155/2013/612848
Research Article

Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation

1Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, China
2Department of Thoracic Cardiovascular Surgery, Affiliated No. 2 Hospital, Zhejiang University, No. 88 Jiefang Road, Hangzhou, Zhejiang 310009, China
3Department of Laboratory Medicine, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, China

Received 5 November 2013; Revised 18 December 2013; Accepted 19 December 2013

Academic Editor: Helen C. Steel

Copyright © 2013 Jiali Tang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Filtration during extracorporeal circulation (ECC) not only removes but also activates leukocytes; therefore, long-term leukocyte filtration may cause adverse effects. In the present study, we tested this hypothesis by priming ECC with 300 mL of canine blood and examining filtration effects in 3 groups ( each) during 60 min ECC. In the control group (Group C) blood was filtrated with an arterial filter for 60 min; in long-term (Group L) and short-term (Group S) groups, blood was filtrated with a leukocyte filter for 60 and 5 min. We found that about 90% of leukocytes were removed after 5 min of filtration in both Groups L and S. Although leukocyte count continued to reduce, mean fluorescent intensities of CD11/CD18, free hemoglobin, and neutrophil elastase increased in Group L and were higher than those in Groups C and S at 60 min. Leukocyte rupture, cytoplasmic leakage, and circulating naked nuclei were also found in Group L. The data support our hypothesis that long-term filtration can induce inflammation and lead to leukocyte destruction.