Figure 6: Immunohistochemical analysis of nesfatin-1 in OA knee cartilage, osteophytes, and synovium under 50× and 200× electron microscope fields. All three tissues expressed nesfatin-1, which was especially high in the lesional areas of cartilage (B > A) and the superficial layers of osteophytes and synovium. Chondrocytes, osteophyte cells, and synoviocytes were strongly stained ((a) damaged cartilage area, (b) nondamaged cartilage area).