Modulation of Conjunctival Goblet Cell Function by Inflammatory Cytokines
Figure 2
Primary mouse goblet cell cultures express goblet cell specific markers. (a) Cells were grown from WT conjunctival explants for 14 days, and the expression of goblet cell specific (CK-7-red and MUC5AC-green) and stratified squamous cell specific (CK-4-red) markers was analyzed by immunofluorescence. Nuclei were counterstained with DAPI (blue). Magnification = ×20. (b) Flow cytometric analysis of goblet cells for the expression of MUC5AC. Unstained cells (empty histogram), isotype controls (filled grey histogram), colonic HT-29 cells (filled green histogram), and cultured goblet cells (filled red histogram).
