Research Article

NAG-1/GDF15 Transgenic Mouse Has Less White Adipose Tissue and a Reduced Inflammatory Response

Figure 6

mice have lower basal and LPS-induced serum leptin concentrations and abdominal fat leptin expression than WT littermates. The serum collected from the terminal bleeds (Figure 5) was analyzed for leptin using a cytokine-specific ELISA. RNA was isolated from the isolated abdominal fat tissue, which was analyzed for relative expression of mouse leptin mRNA using RT-PCR.
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