Minimally Modified LDL Upregulates Endothelin Type A Receptors in Rat Coronary Arterial Smooth Muscle Cells

Table 1

Contractile effects of endothelin-1 (ET-1) in coronary artery.

	𝑛	Endothelin-1
		𝐸max (%)	pEC50
Fresh	8	100±10^∗∗	7.88±0.10^∗∗
24 h culture	8	124±13^∗∗	8.04±0.09^∗∗
24 h culture + mmLDL	8	228±20	8.46±0.08
mmLDL + staurosporine	8	178±18^∗	8.11±0.10^∗
mmLDL + SB386023	8	163±15^∗∗	7.92±0.09^∗∗
mmLDL + U0126	8	156±14^∗∗	8.04±0.11^∗
mmLDL + SP600125	8	192±16	8.37±0.12
mmLDL + SB203580	8	194±18	8.29±0.10
mmLDL + wedelolactone	8	141±13^∗∗	7.96±0.09^∗∗

Responses to endothelin-1 are expressed as E _max in percent of 63.5 mM K⁺-induced contraction and in pEC₅₀ values (negative logarithm of the molar concentration that produces half-maximum contraction). The data are expressed as mean ± SEM. Statistical analyses were performed using two-way ANOVA followed by Dunnett's test and Student's t-test with Welch's correction. 𝑛= number of animals examined in rats. *𝑃<0.05, **𝑃<0.01 versus 24 h culture + mmLDL.