Ox-LDL Promotes Migration and Adhesion of Bone Marrow-Derived Mesenchymal Stem Cells via Regulation of MCP-1 Expression
Figure 6
Role of LOX-1 and MCP-1 in ox-LDL-mediated migration and adhesion of bmMSCs. (a)–(c) Western-blotting assay shows LOX-1, MCP-1 and TGF-β expression in bmMSCs after exposure to 0, 5, 10, and 20 μg/mL ox-LDL for 6 hours. (d) Western-blotting assay shows that LOX-1 antibody inhibits ox-LDL-induced MCP-1 expression. (e) Transwell assay shows that LOX-1 antibody inhibits ox-LDL-induced transmigration of bmMSCs. (f) LOX-1 antibody inhibits ox-LDL-induced cell adhesion between bmMSCs and monocytes. (g) Western-blotting assay shows that LOX-1 antibody decreases ICAM-1 expression. (h) Western-blotting assay shows MCP-1 expression after transfection of noneffective shRNA and MCP-1 shRNA. (i) MCP-1 knockdown inhibits ox-LDL-induced migration of bmMSCs. (j) MCP-1 knockdown inhibits ox-LDL-induced cell-cell adhesion between monocytes and bmMSCs. (k) MCP-1 knockdown decreases ox-LDL-induced ICAM-1 expression. Bar graphs represent mean ± SD ( per group). * versus control, ox-LDL treatment, or negative control transfection.
