Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice
Figure 4
(a) Effects of Nec-1 on ConA-induced hepatic TNF-α and RIP1 protein expression. (b) Caspase 3 activity in cytosolic extracts 12 h after ConA-induced liver injury and pretreatment with vehicle or Nec-1 versus ConA model mice. (c) Localization of IL2 and IL6 in liver tissues detected by immunofluorescence (original magnification: ×200). (d) Immunohistochemical staining for RIP1 and TNF-α after ConA-induced hepatitis in the negative control group (I, IV, VII, and X), the vehicle group (II, V, VIII, and XI), and the Nec-1-treated group (III, VI, IX, and XII). These experiments were repeated three times, and representative results are shown. The small image is ×200 magnification, and the enlarged pictures show the framed parts in the small images (×400).
