Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice
Figure 5
(a) Localization of beclin-1 and LC3 in liver tissues detected with immunofluorescence (original magnification ×200). (b) Immunohistochemical staining of LC3 after ConA-induced hepatitis in the control group, the vehicle-treated group, and the Nec-1-treated group. These experiments were repeated three times, and representative results are shown. The small image is ×200 magnification and the enlarged images show the framed parts of the small images (×400). (c) Effects of Nec-1 on ConA-induced hepatic beclin-1 protein expression. (d) Autophagosome formation induced with ConA is detected in hepatocytes with transmission electron microscopy and was significantly reduced by the Nec-1 intervention. Arrows indicate autophagosomes.
