Cannabinoid Receptor 2 Protects against Acute Experimental Sepsis in Mice
Figure 3
CB2R partially mediates the inhibitory effects of GW405833 on ConA-induced TNF- and IL-6 production in splenocytes. (a)-(b) Splenocytes isolated from CB2R+/+ mice were treated with vehicle or ConA (5 g/mL) in the absence or presence of GW405833 (1–10 M) for 24 h. The levels of IL-6 and TNF- in the supernatants were detected by ELISA. Data are means SD (), *, and ** versus ConA. (c)-(d) Splenocytes isolated from CB2R+/+ and CB2R−/− mice were treated with ConA (5 g/mL) in the absence or presence of GW405833 (10 M) for 24 h. The levels of IL-6 and TNF- in the supernatants were detected by ELISA. Data are means SD (), *, and **.