Alcohol-Induced Liver Injury Is Modulated by Nlrp3 and Nlrc4 Inflammasomes in Mice

Figure 4

Measurement of αSMA and hydroxyproline. Total RNA was isolated from livers of C57BL/6J (B6), Nlrp3^−/−,  and  Nlrc4^−/− mice fed Lieber-DeCarli ethanol-containing diet (+EtOH) or pair-fed controls (Pair-Fed). Expression of (a) αSMA mRNA was measured and normalized by 18S rRNA. (b) Hydroxyproline was measured in protein homogenates by ELISA and normalized by protein concentration in the homogenates. The values are the means ± SEM for 𝑛=4–6 mice per group. Values represent the mean ± SEM with **𝑃<0.05, ***𝑃<0.001 by Student’s 𝑡-test.

(a)

(b)