Alcohol-Induced Liver Injury Is Modulated by Nlrp3 and Nlrc4 Inflammasomes in Mice

Figure 9

Decreased phosphorylation of STAT3 in the liver in Nlrp3^−/− and Nlrc4^−/− mice. Protein was isolated from livers of C57BL/6J (B6), Nlrp3^−/−, and Nlrc4^−/− mice fed Lieber-DeCarli ethanol-containing diet (EtOH-Fed) or pair-fed controls (Pair-Fed). Expression of hepatic total STAT3 and phosphorylated STAT3 was measured by western blot analysis. In the graph, densitometric scans of western blots were performed and analyzed. The values are the means ± SEM. The phosphorylated STAT3 was normalized by total STAT3. Values represent the mean ± SEM with **𝑃<0.05, ***𝑃<0.001 by Student’s 𝑡-test for 𝑛=4–6 mice per group.