Extracellular Signal-Regulated Kinase Is a Direct Target of the Anti-Inflammatory Compound Amentoflavone Derived from Torreya nucifera
Figure 3
Effect of amentoflavone on transcriptional control of inflammatory genes. (a, b, and c) HEK293 or RAW264.7 cells cotransfected with an AP-1-Luc or CREB-Luc plasmid construct (1 μg/mL each) and β-gal (as a transfection control) were treated with amentoflavone (from 0 to 200 μM) in the presence or absence of TNF-α (15 ng/mL), PMA (100 nM), forskolin (2 μM or LPS (1 μg/mL). Luciferase activity was measured using a luminometer. ((d), left panel) Total and phosphorylated levels of the AP-1 family proteins c-Jun and c-Fos in the nuclear fractions of LPS-treated RAW264.7 cells were determined by immunoblotting analyses using specific antibodies. Relative intensity (left panel) was calculated by the DNR Bio-Imaging system (a GelQuant software Ver. 2.7). RI: relative intensity. and compared to the control.
