Research Article
Extracellular Signal-Regulated Kinase Is a Direct Target of the Anti-Inflammatory Compound Amentoflavone Derived from Torreya nucifera
Table 1
Primer sequences used in RT-PCR analysis.
| | Name | | Sequence (5′ to 3′) |
| | Real-time PCR | | | | iNOS | F | GGAGCCTTTAGACCTCAACAGA | | R | TGAACGAGGAGGGTGGTG | | COX-2 | F | CACTACATCCTGACCCACTT | | R | ATGCTCCTGCTTGAGTATGT | | IL-1 | F | GTTGACGGACCCCAAAAAGAT | | R | CCTCATCCTGGAAGGTCCAC | | TNF- | F | TGCCTATGTCTCAGCCTCTT | | R | GAGGCCATTTGGGAACTTCT | | GAPDH | F | CAATGAATACGGCTACAGCAAC | | R | AGGGAGATGCTCAGTGTTGG | | Semiquantitative PCR | | | | iNOS | F | CCCTTCCGAAGTTTCTGGCAGCAG | | R | GGCTGTCAGAGCCTCGTGGCTTTGG | | TNF- | F | TTGACCTCAGCGCTGAGTTG | | R | CCTGTAGCCCACGTCGTAGC | | COX-2 | F | CACTACATCCTGACCCACTT | | R | ATGCTCCTGCTTGAGTATGT | | GAPDH | F | CACTCACGGCAAATTCAACGGCA | | R | GACTCCACGACATACTCAGCAC |
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