Cigarette Smoke Suppresses the Surface Expression of c-kit and FcεRI on Mast Cells
Figure 3
Long-term exposure of mast cells to CSM inhibits allergic degranulation. BMMCs were cultured in regular culture medium, in the presence of CSM (1.5%) or LPS (100 ng/mL) in the third week of bone marrow culture. Then, cells were sensitized with DNP-specific IgE, followed by activation with dinitrophenyl-conjugated human ovalbumin (DNP-Ova). Degranulation was assessed by the release of -hexosaminidase in the supernatants from cells. Data are mean ± SEM of quadruplicate samples ().
