Contribution of CFTR to Alveolar Fluid Clearance by Lipoxin A4 via PI3K/Akt Pathway in LPS-Induced Acute Lung Injury
Figure 5
LPS decreases protein expression of CFTR via PI3K/Akt signaling pathway in ATII cells. The cells were incubated with LPS (1 μg/mL) for 10, 30, 60, 120, and 240 min. After incubation, the cells were harvested and sonicated. (a) Phosphorylation of Akt and ERK in the cell lysates were assessed by western blotting. (b) The rat primary ATII cells were pretreated with LY294002 (10 μM, PI3K/Akt inhibitor) or U0126 (20 μM, ERK inhibitor) for 30 min and then incubated with LPS (1 μg/mL) for 6 h. After incubation, the cells were harvested, sonicated and CFTR protein detected by western blotting. Data were expressed as mean ± SEM for each group. * versus control group, # versus LPS group, for each group. LPS = lipopolysaccharide, CFTR = cystic fibrosis transmembrane conductance regulator.
