The Role of T Cell Immunoglobulin Mucin Domains 1 and 4 in a Herpes Simplex Virus-Induced Behçet’s Disease Mouse Model

Figure 9

The frequencies of Treg cells were upregulated after treatment with siTim-4 in Behçet’s disease (BD) mice. Treg cells were confirmed by flow cytometry in lymph nodes of BD mice after treatment with siTim-4. At 1 and 2 weeks after the first treatment with siTim-4, the frequencies of CD4⁺CD25⁺, CD4⁺Foxp3⁺, and CD4⁺CD25⁺Foxp3⁺ Treg cells were compared between the siTim-4 and control groups (negative control siRNA treated groups) (𝑛=5–7) (**𝑃<0.05). The bottom panel shows CD4⁺CD25⁺ in PBMCs at 2 weeks by FACS dot plot. Con: negative control siRNA injection to BD mice, siTim-4: Tim-4 siRNA injection to BD mice.