Nitric Oxide Is a Mediator of Antiproliferative Effects Induced by Proinflammatory Cytokines on Pancreatic Beta Cells
Figure 2
Effect of NO inhibition on islet β-cell proliferation. Rat islets were cultured for 48 h in the presence of cytokines IL-1β (50 U/mL) + IFN-γ (1000 U/mL) + TNF-α (1000 U/mL) (CTKS) alone or in combination with NOS inhibitor L-NMMA and stained with BrdU over the course of the culture period. Results are presented as percentage means ± SEM of combined BrdU/insulin positive cells relative to insulin-positive cells in a minimum of 5 experiments. treated versus control.
