Activation of TRPV1 Prevents OxLDL-Induced Lipid Accumulation and TNF-α-Induced Inflammation in Macrophages: Role of Liver X Receptor α
Figure 1
Expression of TRPV1 is increased in atherosclerotic lesions of ApoE−/− mice. (a) Western blot analysis of protein expression of TRPV1 in aortas from 5-month-old ApoE−/− and wild-type (WT) mice. α-Tubulin was a normalization control. Data are mean ± SD from 6 animals. * versus WT mice. (b) Immunohistochemical staining for TRPV1, F4/80 (macrophage marker), and α-actin (smooth-muscle-cell marker) in atherosclerotic lesions of aortas from 5-month-old ApoE−/− mice. Specificity of immunostaining was confirmed with an IgG-negative control. Hematoxylin was used as counterstaining. Magnification = 100 x. (c) Western blot analysis of protein expression of TRPV1 induced by oxLDL (50 μg/mL) relative to that induced by vehicle (PBS) for 0–24 h. Data are mean ± SD from 5 independent experiments. * versus vehicle-treated group. α-Tubulin was a normalization control.
